Keane, KM, Salicki, R, Goodall, S, Thomas, K, and Howatson, G. Muscle damage response in female collegiate athletes after repeated sprint activity. J Strength Cond Res 29 (10): 2802–2807, 2015—Exercise-induced muscle damage (EIMD) is a well-investigated area, however there is a paucity of data surrounding the damage response in females. The aim of this study was to examine the damage responses from a sport-specific bout of repeated sprints in female athletes. Eleven well-trained females (mean±SD; age: 22±3 years; height: 166.6±5.7 cm; mass: 62.7±4.5 kg) in the luteal phase of the menstrual cycle completed a repeated sprint protocol designed to induce EIMD (15× 30 m sprints). Creatine kinase, countermovement jump height, knee extensor maximal voluntary isometric contraction (MVIC) force, delayed onset muscle soreness (DOMS), 30-m sprint time, and limb girth were recorded before, after, 24, 48, and 72 hours after exercise. Creatine kinase was elevated at 24, 48, and 72 hours (p≤ 0.05), peaking at 24 hours (+ 418%) and returning toward baseline at 72 hours. Countermovement jump height was reduced immediately after, 24, and 48 hours (p≤ 0.05). Sprint performance was also negatively affected immediately after, 24, 48, and 72 hours after exercise. Muscle soreness peaked at 48 hours (p< 0.01) and remained significantly elevated at 72 hours after exercise (p< 0.01). Limb girth and MVIC did not alter over time. This study provides new information on the EIMD response in trained females after a sport-specific bout of repeated sprints. Importantly, this damage response has the potential to negatively affect performance for several days after exercise.